Alendronate (ALD), when administered orally, has several limitations, including poor bioavailability and gastrointestinal irritation. This study addresses the need for a sustained local delivery system for ALD by fabricating Hydroxyapatite (HA) hollow microspheres using a simple template-directed method with mustard seeds as the template. ALD was adsorbed onto the surface of the HA hollow microspheres at varying concentrations (0.05 µM to 0.2 µM). The microsphere characteristics properties of the surface and fundamental makeup were examined using a combination of functional group characteristics by Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and high energetic powder X-ray studies. The cytotoxic effects of the ALD-HA composite were evaluated In vitro using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay on fibro sarcoma HTT-1080 cells. Additionally, the drug release capacity of the hollow microspheres was assessed. The findings demonstrate that the hollow ALD-HA microspheres were successfully fabricated and also are a promising desirable action at local sites and designed for bisphosphonates for the management of osteoporosis.
