Melissa officinalis and Stachys ocymastrum are aromatic and therapeutic plants from the Lamiaceae family. This study aimed to analyze the phytochemical content and antioxidant and antibacterial properties of acetone and methanol extracts of M. officinalis and S. ocymastrum. The quantification included polyphenols, flavonoids, condensed tannins, flavonols, and proanthocyanidins. Antioxidant activities were evaluated through DPPH radical scavenging, ferric-reducing power, and ferrous chelation tests. Antibacterial effects were assessed using disk diffusion and microdilution methods. The findings revealed significant levels of polyphenols (ranging between 94.05 and 15.54 mg EGA/g DW), flavonoids, tannins, flavonols, and proanthocyanidins in both M. officinalis and S. ocymastrum extracts. Therefore, the two plants exhibited a notable ability to combat free radicals, reduce, and chelate iron, displaying their potent antioxidant properties. However, S. ocymastrum has significant DPPH˙ free radical scavenging activity (IC50 values: 0.06 mg/mL, 0.08 mg/mL, and 0.09 mg/mL, respectively). Moreover, both plants displayed substantial activity against Gram-positive and Gram-negative bacterial strains, underscoring M. officinalis and S. ocymastrum's potential as effective antibacterial agents. This plant holds promise for future advancements in medicine and nutrition, reinforcing its traditional use as a health-promoting herbal infusion.
