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2024 Volume 15 Issue 2

Fatty Acid Composition in Bone Fluid from Knee Osteoarthritis Patients


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  1. Laboratory of Biophysics and Medical Technologies, Higher Institute of Medical Technologies of Tunis, University of Tunis El Manar, Tunisia.
  2. Laboratory of Valorization of Useful Materials, National Center for Research in Materials Science, Technologic Park of Borj Cedria, BP73, 8028 Soliman, Tunisia.
  3. Laboratory of Biophysics and Medical Technologies, Higher Institute of Medical Technologies of Tunis, University of Tunis El Manar, Tunisia.
Abstract

Recently, the use of bone grafts increased in medical practice. Some bone grafts may have different mechanical and biological properties of natural bone. To improve bone grafts we need to record our knowledge about bone. In this work, we are interested in determining the chemical composition of bone fluid in the knee joint as it is a lipid-predominant fluid. Bone samples were obtained from knee cancellous bone. Samples were used of women aged 60 years old with osteoarthritis. Sample preparation is essential to guarantee the quality and dependability of the analysis results. Bone fluid was centrifuged for 15 minutes at 3000 rpm to eliminate the blood and debris. The supernatant was then gathered and filtered. Extraction of total lipids from the bone fluid according to the method of Bligh and Dyer (1959) with modification. The bone fluid of a fresh human cancellous bone was extracted by mechanical compression tests. It consists of 90% lipids. The fatty acid composition of the bone fluid was determined by gas chromatography. Methyl oleate is the most abundant.


How to cite this article
Vancouver
Benhmida S, Trabelsi H. Fatty Acid Composition in Bone Fluid from Knee Osteoarthritis Patients. J Biochem Technol. 2024;15(2):23-6. https://doi.org/10.51847/UNhuTqup51
APA
Benhmida, S., & Trabelsi, H. (2024). Fatty Acid Composition in Bone Fluid from Knee Osteoarthritis Patients. Journal of Biochemical Technology, 15(2), 23-26. https://doi.org/10.51847/UNhuTqup51
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JOURNAL OF BIOCHEMICAL TECHNOLOGY
JOURNAL OF BIOCHEMICAL TECHNOLOGY
Journal of Biochemical Technology is a double-blind peer reviewed International Journal published by the Deniz Publication on behalf of the Biochemical Technology Society, a Registered Charity Organization from India

AREA OF INTEREST
AREA OF INTEREST
new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.

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FOCUS AND SCOPE
Journal of Biochemical Technology provides a publication on all aspects of biochemistry, biotechnology & bioinformatics and applications in biology and medicine. Areas of high interest cover new advances in enzymatic and protein mechanisms; applied molecular genetics and biotechnology; computational biology, genomics and proteomics; metabolic & tissue engineering; medical, environmental, Pharmacy and pharmaceutical chemistry, food and agro-biotechnology.

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