Natural and sustainable alternatives are becoming more and more popular as people become more conscious of the negative impacts that synthetic chemicals have on their health and the environment. Making soap from plant extracts became more popular in these circumstances. The current study aims to characterise and investigate the physico-chemical and antimicrobial properties of synthesized natural soaps. The properties such as pH, volatile matter, total fatty matter, and antibacterial activity of the soaps were measured and compared to those of commercial soaps that are available in the market. The agar-disc diffusion method was used to evaluate the antimicrobial efficacy of these natural soaps against certain microorganisms. According to results, soap types such as neem and beetroot (NB) soap, sandal and turmeric (ST) soap, commercial soap 1 (CS1), commercial soap 2 (CS2), and commercial soap 3 (CS3) had shown significant zones of inhibition. Some soap types did not exhibit any inhibitory zone against certain microorganisms. As per the results, it was concluded that the soaps made from neem and beetroot (NB) and sandal and turmeric (ST) had high antimicrobial qualities, effective alkaline pH (9.3), less volatile matter (6%), low alkaline content (3.5), and grade 1 total fatty matter (77%). As a result, both soaps are amenable to industrial production.