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2022 Volume 13 Issue 1

Immobilization and Performance of Cellulase on Recyclable Magnetic Hydrotalcites


Tran Boi An, Duong Huynh Thanh Linh, Nguyen Phung Anh, Tran Thi Tuong An, Nguyen Tri
Abstract

mHT(Zn) and mHT(Mg) hydrotalcites were fabricated by co-precipitation of Zn2+/Al3+ and Mg2+/Al3+ salt mixtures in the presence of Fe3O4 and used as supports for immobilizing cellulase to form [email protected](Zn) and [email protected](Mg). The structure and properties of mHT(Zn), mHT(Mg), [email protected](Zn), and [email protected](Mg) were characterized by Fourier−transform infrared spectroscopy, X-ray diffraction, filtering electron microscopy. The effect of pH, cellulase concentration, and the number of supports on the immobilization of cellulase onto supports were carefully investigated. The enzyme activity of free cellulase, immobilized cellulase, and immobilization efficiency was analyzed by determining reduced glucose using DNS as a color indicator. The highest immobilization efficiency obtained was 94.9 % when carried out on mHT(Zn) at pH 6.5 and 95.3 % on mHT(Mg) and the concentration of cellulase in 0.1mg/mL at the pH of 5.5, using 0.2 g of supports. [email protected](Zn) and [email protected](Mg) show high enzyme activity when reacting with 1 % CMC solution at 50 oC with relative enzyme activity of 78.0% and 70.4 %, respectively.


How to cite this article
Vancouver
An TB, Linh DHT, Anh NP, An TTT, Tri N. Immobilization and Performance of Cellulase on Recyclable Magnetic Hydrotalcites. J Biochem Technol. 2022;13(1):13-9. https://doi.org/10.51847/APmQMAcejg
APA
An, T. B., Linh, D. H. T., Anh, N. P., An, T. T. T., & Tri, N. (2022). Immobilization and Performance of Cellulase on Recyclable Magnetic Hydrotalcites. Journal Of Biochemical Technology, 13(1),13-19. https://doi.org/10.51847/APmQMAcejg
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JOURNAL OF BIOCHEMICAL TECHNOLOGY
JOURNAL OF BIOCHEMICAL TECHNOLOGY
Journal of Biochemical Technology is a peer reviewed International Journal published by the Sevas Publishing on behalf of the Biochemical Technology Society, a Registered Charity Organization from India

AREA OF INTEREST
AREA OF INTEREST
Areas of high interest, cover new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.

FOCUS AND SCOPE
FOCUS AND SCOPE
Journal Of Biochemical Technology” Provides A Medium For The Rapid Publication Of Full-Length Articles, Mini-Reviews Of New And Emerging Products And Short Communications On All Aspects Of ...

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This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. Keywords include, Biochemical Research: Endo/exocytosis, Trafficking, Membrane Biology, Cell Migration, Cell-Matrix Organelle Biogenesis, Cytoskeleton Proteolysis, Cell Death, Cell Cycle, Cancer, Cell Growth/Death, Differentiation, Drug Targets, Gene Therapy, Models of Disease, Proteomics, Stem Cells, Bioenergetics, Mitochondria, Free Radicals, Redox Signaling, Ion Transport/Channels, Oxidative