TY  - JOUR
T1  - Immobilization and Performance of Cellulase on Recyclable Magnetic Hydrotalcites
A1  - Tran Boi An
A1  - Duong Huynh Thanh Linh
A1  - Nguyen Phung Anh
A1  - Tran Thi Tuong An
A1  - Nguyen Tri
JF  - Journal of Biochemical Technology
JO  - J Biochem Technol
SN  - 0974-2328
Y1  - 2022
VL  - 13
IS  - 1
DO  - 10.51847/APmQMAcejg
SP  - 13
EP  - 19
N2  - mHT(Zn) and mHT(Mg) hydrotalcites were fabricated by co-precipitation of Zn2+/Al3+ and Mg2+/Al3+ salt mixtures in the presence of Fe3O4 and used as supports for immobilizing cellulase to form cell@mHT(Zn) and cell@mHT(Mg). The structure and properties of mHT(Zn), mHT(Mg), cell@mHT(Zn), and cell@mHT(Mg) were characterized by Fourier−transform infrared spectroscopy, X-ray diffraction, filtering electron microscopy. The effect of pH, cellulase concentration, and the number of supports on the immobilization of cellulase onto supports were carefully investigated. The enzyme activity of free cellulase, immobilized cellulase, and immobilization efficiency was analyzed by determining reduced glucose using DNS as a color indicator. The highest immobilization efficiency obtained was 94.9 % when carried out on mHT(Zn) at pH 6.5 and 95.3 % on mHT(Mg) and the concentration of cellulase in 0.1mg/mL at the pH of 5.5, using 0.2 g of supports. Cell@mHT(Zn) and cell@mHT(Mg) show high enzyme activity when reacting with 1 % CMC solution at 50 oC with relative enzyme activity of 78.0% and 70.4 %, respectively. 
UR  - https://jbiochemtech.com/article/immobilization-and-performance-of-cellulase-on-recyclable-magnetic-hydrotalcites-9rnf5cedw0053r2
ER  -