Written by Zulfiqar Ali Raza and Aisha Rehman
Published On Issue 2 Vol 7, 2016
The effect of Fe+2, Mg+2, Ca+2 cations, and sodiumdodecylsulphate (SDS) and cetyltrimethlyammonium bromide (CTAB) surfactants was investigated on amylase activity in the aqueous media. Three commercially available enzymes (Luzyme HP, Aquazym SDL, Bactosol PHC) were used for this purpose under various setups. In the first setup, individual effect of metal ions and surfactants was studied, and then based on the results from first setup; in the second setup, trend of amylase activity was studied
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Written by Kajal G Singh
Published On Issue 2 Vol 7, 2016
Plackett-Burman and Response Surface Methodology were used to optimize fermentation medium for enhancing butanol production by Clostridium beijerenckii strain CHTa. Optimization using Plackett-Burman design showed yeast extract, MgSO4.7H2O and glucose to have signiï¬cant impact over other factors, namely (NH4)2SO4, CaCO3, FeSO4.7H2O, cysteine hydrochloride, sodium thioglycolate and pH. To find out the optimal concentrations and interactions between the selected factors, further experiments were
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