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2016 Volume 7 Issue 2

Optimization for butanol production using Response Surface Methodology by Clostridium beijerenckii strain CHTa isolated from distillery waste manure.


Kajal G Singh, Kaushal L Lapsiya, Rekha R Gophane, Dilip R Ranade
Abstract
Plackett-Burman and Response Surface Methodology were used to optimize fermentation medium for enhancing butanol production by Clostridium beijerenckii strain CHTa. Optimization using Plackett-Burman design showed yeast extract, MgSO4.7H2O and glucose to have significant impact over other factors, namely (NH4)2SO4, CaCO3, FeSO4.7H2O, cysteine hydrochloride, sodium thioglycolate and pH. To find out the optimal concentrations and interactions between the selected factors, further experiments were carried by applying Central Composite Design. The experimental results demonstrated glucose and MgSO4.7H2O as the key components over yeast extract responsible for production of butanol by strain CHTa. A mathematical model was established to predict the optimum values for factors. Under optimal conditions, practical butanol yield of 10.08 g L-1 and productivity of 0.45 g L-1 h-1 were obtained.

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JOURNAL OF BIOCHEMICAL TECHNOLOGY
JOURNAL OF BIOCHEMICAL TECHNOLOGY
Journal of Biochemical Technology is a double-blind peer reviewed International Journal published by the Sevas Publishing on behalf of the Biochemical Technology Society, a Registered Charity Organization from India

AREA OF INTEREST
AREA OF INTEREST
new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.

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FOCUS AND SCOPE
Journal Of Biochemical Technology Provides A Medium For The Rapid Publication Of Full-Length Articles, Mini-Reviews Of New And Emerging Products And Short Communications On All Aspects Of ...

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This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. Keywords include, Biochemical Research: Endo/exocytosis, Trafficking, Membrane Biology, Cell Migration, Cell-Matrix Organelle Biogenesis, Cytoskeleton Proteolysis, Cell Death, Cell Cycle, Cancer, Cell Growth/Death, Differentiation, Drug Targets, Gene Therapy, Models of Disease, Proteomics, Stem Cells, Bioenergetics, Mitochondria, Free Radicals, Redox Signaling, Ion Transport/Channels, Oxidative