In this work, kinetic properties evaluation was made for bioethanol production from sugar hydrolysate of vegetable waste. The saccharified sugars were fermented by using Saccharomyces cerevisiae. The effect of various saccharification factors on sugars release were studied and observed that the optimized conditions contributed to 14.4 gL-1of fermentable sugars production. The produced sugars were subjected to batch fermentation by Saccharomyces cerevisiae at pH 4.5 and the kinetic parameters of
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p { margin-bottom: 0.21cm; }We investigated the neuroprotective effect of Neurotol (Mannitol 10% + Glycerin 10% + RD011 1.5 %) against neuronal damage induced by glutamate compared to Neurotol M (Mannitol 20%), Kratol and Zemisol. Exposure of neuronal cells to glutamate caused concentration dependent neuronal damage as evident by significant increase in LDH activities (P<0.05 to P<0.001). The neuronal cell toxicity by exposure to glutamate involves an apoptotic process. However, among the
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Natural β-carotene, an orange–red pigment of carotenoid family is widely used as food colorant. In this study microbial method of pigment production is adopted to encompass the other expensive technologies. Influence of substrate (rice powder) on β-carotenoid production has been extensively studied at optimum process conditions such as pH, temperature etc. A novel β-carotenoid producing pigment Bacillus clausii was isolated and identified by physiological tests as well as 16s rDNA sequence
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This paper is a comparative study of phenol biodegradation by soluble and alginate entrapped turnip (Brassica rapa) peroxidase. The effects of relevant factors on the process such as pH, temperature, concentration of H2O2, phenol concentration, enzyme activity and contact time were evaluated in order to optimize the conditions for maximum phenol removal. Results showed that the obtained average removal yield under optimal conditions was 93%. The process duration was 3 hours. The reac
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There is an increasing interest in identifying naturally occurring potent preventive and therapeutic agents for cancer. Shemamruthaa, a phytochemical formulation was evaluated for the first time with a view to potentiate more intense anticancer property. Adult female Sprague-Dawley rats (8-week-old) were used for the study and were divided into 4 Groups. Group I control animals received standard pellet diet and water ad libitum. Group II animals were induced for mammary carcinoma with a single o
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Nineteen bacterial strains were isolated from vermisources andscreened for Indole-3-acetic acid (IAA) production among themonly nine strains produce IAA and they were identified asStreptococcus spp., Micrococcus spp., Klebsiella spp., Bacillus spp., Enterobacter spp., Escherichia spp., Alcaligenes spp., Erwinia spp., and Pseudomonas spp. Among all other strains Bacillus sp. showed the higher IAA production hence selected for further molecular analysis and confirmed as Bacillus cereus. The B. cer
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Ten different thermotolerent bacteria were isolated from terrestrial thermal spring of Unkeshwar in Nanded district of Maharashtra (India). These isolates were characterized by morphological characters, microscopic features, biochemical pattern and physiological attributes. These isolates were identified as Bacillus licheniformis (APP7), Bacillus megaterium (APP8), Actinobacillus hominis (APP9), Lysinibacillus sphaericus (APP10), Paenibacillus alvei (APP11), Bacillus simplex (APP12), Actin
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Saccharomyces cerevisiae (NCIM 3287) was immobilized in sodium alginate beads to produce a biocatalyst for use in guava wine making. The immobilized biocatalyst was found to be suitable for guava juice fermentation at ambient temperatures. The study included effects of alginate concentrations, initial bead loadings and bead diameters on fermentation. The optimized parameters were 2% (w/v) alginate concentration, 60g/l initial bead loading and  
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Pseudomonas sp. GBS.5 is a newly isolated biosurfactant producing and carbazole degrading bacterium. In the present study, this bacterium was coated with magnetite nanoparticles, synthesized using co-precipitation method. Scanning electron microscopy (SEM) studies confirmed the coating of the bacterial surface with these nanoparticles. Degradation activity of the coated cells obtained was 1.4 ppm/min as compared to 0.32 ppm/min for free cells and could be reused for five different cycles. These
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Oxalate decarboxylase (OxDC), an enzyme of the bicupinsuperfamily, catalyzes the decomposition of oxalate into carbondioxide and formate at an optimal pH of 4.3 in the presence ofoxygen. However, about 0.2% of all reactions occur through anoxidase mechanism that consumes oxygen while producing twoequivalents of carbon dioxide and one equivalent of hydrogenperoxide. The kinetics of oxidase activity were studied bymeasuring the consumption of dissolved oxygen over time using a luminescent oxygen s
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The present investigations influence of Penicillium species and their culture filtrates on the seed germination and seedling growth of sorghum was studied. Culture filtrate of P. nordicum, P. verrucosum, P. chrysogenum, P. commune, P. citrinum, P.camemberti and P. digitatum were comparatively more toxic and significantly inhibited the seed germination and seedling growth varied with the species. However, pathogenicity of different inoculum of Penicillium species also exhibited varying seedling g
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Nowadays nanoparticles are widely used as a key tool for enzyme immobilization. Tropizyme-P, a pectolytic enzyme was successfully immobilized on amino functionalized magnetic nanoparticles (AMNPs) using glutaraldehyde as a cross-linking agent at 15 mM concentration and 4h cross-linking time. The average size of the synthesized AMNPs was found below 80 nm by particle size analyzer. The binding of tropizyme-P on nanoparticles was confirmed by FTIR spectroscopy. SEM analysis revealed that there was
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Journal of Biochemical Technology is a double-blind peer reviewed International Journal published by the Deniz Publication on behalf of the Biochemical Technology Society, a Registered Charity Organization from India
new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.
Journal of Biochemical Technology provides a publication on all aspects of biochemistry, biotechnology & bioinformatics and applications in biology and medicine. Areas of high interest cover new advances in enzymatic and protein mechanisms; applied molecular genetics and biotechnology; computational biology, genomics and proteomics; metabolic & tissue engineering; medical, environmental, Pharmacy and pharmaceutical chemistry, food and agro-biotechnology.
