Yeastolate is an efficient serum replacement to fully support insect cell growth and baculovirus replication in the insect cell/baculovirus expression system. However, yeastolate is an undefined complex substance and therefore subject to batch-to-batch variation, which often causes inconsistent cell growth and productivity. Therefore, the present study aims to identify yeastolate components that promote insect cell growth and baculovirus production. Gel filtration chromatography was used to dete
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This study sought to asses and characterizes the inhibitory action of methanolic extract of Pithecellobium dulce (P. dulce) seeds on α-amylase and α-glucosidase enzymes as well as to characterize compounds responsible for these activities. The methanolic extract was assessed for total phenolic, flavanoid and triterpenoids content by using Folin-Ciocalteu’s reagent, Aluminum chloride (AlCl3) and Vaniline-perchloric acid assay, respectively. The methanolic extract was further qua
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Medicinal plants and their phytochemical constituents are considered as paramount entity to treat various human ailments. The present study was designed to investigate the effect of ethanolic extract of Hygrophila auriculata on mercury induced oxidative damage in rat liver. Mercuric chloride (HgCl2) (1mg/kg b.w, i.p) was administered three times in a week for two weeks to induce mercury toxicity. Mercury damage to the liver was evident from increase in the activities of marker enzymes such as al
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Investigations were conducted to determine the proteolytic activity using bromocresol green dye on substrate agar plates. Two methods were highlighted in this article. The first method was adopted to determine the proteolytic activity by flooding bromocresol green reagent on casein/skimmed milk agar plates. Later, a minimum of 0.0015% of bromocresol green was incorporated with the substrate agar plates before autoclaving to detect the proteolytic activity of bacteria. The proteolytic activity ap
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