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2013 Volume 4 Issue 3

Identification of bioactive yeastolate fractions responsible for insect cell growth and baculovirus production


Abstract
Yeastolate is an efficient serum replacement to fully support insect cell growth and baculovirus replication in the insect cell/baculovirus expression system. However, yeastolate is an undefined complex substance and therefore subject to batch-to-batch variation, which often causes inconsistent cell growth and productivity. Therefore, the present study aims to identify yeastolate components that promote insect cell growth and baculovirus production. Gel filtration chromatography was used to determine the molecular weight profiles of yeastolate and fresh/spent culture media during Sf-21 insect cell growth and infection. By comparing the differences among these molecular weight profiles, yeastolate was fractionated into five different molecular weight ranges of fractions. Yeastolate fraction with molecular weight below 1 kD was found to contain bioactive components responsible for the cell growth, but the ingredients essential for baculovirus production were localized in yeastolate fraction between 1 to 3 kD. These results indicate that nutrient utilization was significantly different during the cell growth and infection. The present study provides a high-resolution guideline for further identification of specific bioactive ingredients in yeastolate to develop fully chemically-defined serum-free media for the insect cell/baculovirus system.

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JOURNAL OF BIOCHEMICAL TECHNOLOGY
JOURNAL OF BIOCHEMICAL TECHNOLOGY
Journal of Biochemical Technology is a double-blind peer reviewed International Journal published by the Sevas Publishing on behalf of the Biochemical Technology Society, a Registered Charity Organization from India

AREA OF INTEREST
AREA OF INTEREST
new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.

FOCUS AND SCOPE
FOCUS AND SCOPE
Journal Of Biochemical Technology Provides A Medium For The Rapid Publication Of Full-Length Articles, Mini-Reviews Of New And Emerging Products And Short Communications On All Aspects Of ...

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This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. Keywords include, Biochemical Research: Endo/exocytosis, Trafficking, Membrane Biology, Cell Migration, Cell-Matrix Organelle Biogenesis, Cytoskeleton Proteolysis, Cell Death, Cell Cycle, Cancer, Cell Growth/Death, Differentiation, Drug Targets, Gene Therapy, Models of Disease, Proteomics, Stem Cells, Bioenergetics, Mitochondria, Free Radicals, Redox Signaling, Ion Transport/Channels, Oxidative