Surface charge, molecular weight, and folding state are known to influence protein chromatographic behavior onto ion-exchangers. Experimentally, information related to such factors can be gathered via two-dimensional electrophoretic (2-DE) methods. The separation behavior depicted by the insect cultured-cells proteome, which is an important host for recombinant protein production, was explored in this study. Experimental evidence showed a correlation between apparent isoelectric point distributi
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The fundamental mechanism of olfactory receptor activation has been conserved from yeast to humans. Engineered yeast cells can smell some of the same odorants as humans can, which makes yeast an ideal model system for studying human olfaction. Furthermore, if engineered yeast cells are incorporated into sensory arrays, they can be used as biosensors or artificial noses.Keywords: Yeast, olfactory receptor, G protein-coupled receptor, biosensor, smellReceived: 31 July 2008 / Received in revised fo
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BRCA1 and DNA-PK are two significant multifunctional proteins involved primarily in the processing of double strand breaks (DSBs). BRCA1 participates actively in homologous recombination (HR) while DNA-PK in non-homologous end joining (NHEJ). In this mini review, we discuss all recent evidence for a possible involvement of these repair proteins also in the processing of oxidatively-induced DNA damage.Keyword: DNA damage, BRCA1, DNA-PKReceived: 6 June 2008, Accepted: 10 August 2008 Published onli
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To evaluate the effectiveness of T-DNA insertion in knocking out a gene, 1084 published Arabidopsis thaliana insertion mutants representing 755 genes in 648 publications were reviewed. Insertion in the protein-coding region of a gene generates a knockout at least 90% of the time or 25% of the time if it is before the start codon which also produces a knockdown 67% of the time. Insertion after the stop codon had no effect on transcription of the upstream gene 17% of the time compared to 8% in ins
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Abstract not available! Keyword: Hatching enzyme, volvox, evolution, multicellularityReceived: 6 June 2008, Accepted: 10 August 2008, Available online: 26 August 2008
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This paper provides an introduction to the concept and importance of journal impact factor (JIF), citation indexes. The paper takes the form of a short history of JIF, the importance of journal performance indicators, about calculating and determining JIF and the significance of using Citation Indexes. Elements of the factors affecting impact factor namely scientometrics and journalology were also highlighted. A guide to locating the best impact factor journals in various research fields was dem
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“Recent abstracts in biochemical technology†is a collection of interesting research articles published in “List of biochemical technology journals†(Table 1). The abstracts are most likely to report significant results in biochemical technology.
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