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2008 Volume 1 Issue 1

How effective is T-DNA insertional mutagenesis in Arabidopsis?


Abstract
To evaluate the effectiveness of T-DNA insertion in knocking out a gene, 1084 published Arabidopsis thaliana insertion mutants representing 755 genes in 648 publications were reviewed. Insertion in the protein-coding region of a gene generates a knockout at least 90% of the time or 25% of the time if it is before the start codon which also produces a knockdown 67% of the time. Insertion after the stop codon had no effect on transcription of the upstream gene 17% of the time compared to 8% in insertion before the start codon. T-DNA insertion can also cause deletion and translocation.Keywords: Arabidopsis thaliana, deletion, expression, knockout, T-DNA insertion, translocationReceived: 2 June 2008 / Received in revised form: 13 June 2008, Accepted: 20 June 2008, Published online: 18 August 2008

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JOURNAL OF BIOCHEMICAL TECHNOLOGY
JOURNAL OF BIOCHEMICAL TECHNOLOGY
Journal of Biochemical Technology is a peer reviewed International Journal published by the Sevas Publishing on behalf of the Biochemical Technology Society, a Registered Charity Organization from India

AREA OF INTEREST
AREA OF INTEREST
Areas of high interest, cover new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.

FOCUS AND SCOPE
FOCUS AND SCOPE
Journal Of Biochemical Technology” Provides A Medium For The Rapid Publication Of Full-Length Articles, Mini-Reviews Of New And Emerging Products And Short Communications On All Aspects Of ...

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This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. Keywords include, Biochemical Research: Endo/exocytosis, Trafficking, Membrane Biology, Cell Migration, Cell-Matrix Organelle Biogenesis, Cytoskeleton Proteolysis, Cell Death, Cell Cycle, Cancer, Cell Growth/Death, Differentiation, Drug Targets, Gene Therapy, Models of Disease, Proteomics, Stem Cells, Bioenergetics, Mitochondria, Free Radicals, Redox Signaling, Ion Transport/Channels, Oxidative