The determination of the frequency of HDFN found in jaundiced newborns in Sudan was the goal of the analysis. In Omdurman maternity inside a Sudanese hospital, 220 mothers and their babies who had jaundice altogether were received in hospital-Sudan and a prospective cross-sectional hospital-based analysis was carried out them. In addition to ABO and Rhesus blood group typing, indirect antiglobulin test (IAT) and direct coomb's test (DAT) was applied for mothers and their jaundiced newborns, respectively. IgG anti-A and anti-B were checked inside the infant’s plasma to detect any incompatibility of ABO. The infant’s blood samples were taken and it underwent the tests of Hemoglobin, Reticulocyte count, thin blood films, and packed cell volume to check the hemolytic status of the baby. The history of abortion, prophylactic anti-D and blood transfusion was written as well. From the analysis, 57.3% (63/110) was the fetus’s and newborn's frequency of hemolytic disease among the studied subjects. The ones with incompatibility of ABO are 28.6% (18/63), and the ones with incompatibility of Rh (D) are 20.6% (13/63) even while prophylactic anti-D was used, while 50.8% (32/63) as a result from other blood groups antigens. The study concluded that the most common cause of HDFN in the studied population is from the other blood group antigens. The analysis also showed that the happening of Rh (D) HDFN wasn’t erased in spite of the Rh immunoglobulin usage.