The goal of this study was to see how ZnNPs (a green synthesis formulation) affected the physiological, biochemical, and oxidative stress changes generated by experimental diabetes in rats. Twenty female albino Wistar rats were randomly separated into four groups (n=5): control, zinc nanoparticles, diabetic rats, and diabetic rats treated with zinc nanoparticles. Rats were given all types of treatments orally for 21 days. Alloxan (150mg/kg) was used to make rats diabetic. Various parameters were calculated, including biochemical, hematological, and oxidative stress markers. Histopathological lesions of pancreas tissues were noticed. Characterizations of nanoparticles were analyzed using standard technics. UV-Vis Spectroscopy revealed that ZnNPs exhibit a peak at 230 nm, and SEM indicated that the produced ZnNPs are a smaller size than 21.5 nm. The FTIR spectra revealed a peak in the range of 400-700 cm-1. In vivo results revealed a change in the diabetic group's lipid profile and metabolic markers. While the liver, kidneys, and pancreas antioxidant defense systems were harmed, by increasing MDA levels and GSH levels and SOD activity are declining compared to control. Furthermore, hematological measurements demonstrated that diabetes reduced RBC, MCV, and HCT levels significantly (P<0.01). When diabetic rats' pancreas tissues were compared to control rats', histopathological examination revealed a difference. On the other hand, treating diabetic rats with ZnNPs), improved and corrected partially of the prior parameters. Finally, the utilization of ZnNPs synthesized by polyherbal extract appears to be the most important limiting factor in diabetes development and consequences.
