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2023 Volume 14 Issue 2

Antimicrobial Potential of Azadirachta indica (Neem) Leaves Extracts Against Various Pathogenic Bacteria


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Abstract

Antimicrobial resistance has become a serious threat to human health nowadays. This research aimed to explore the potential of plants to be used as antimicrobial agents against pathogenic bacteria. Three different extracts of Azadirachta indica (Neem) leaves (methanolic, ethanolic, and aqueous) were tested against 5 Gram-positive and 4 Gram-negative bacteria. For Gram-positive bacteria, ethanolic extract showed the best antimicrobial potential. Bacillus cereus was found more sensitive to ethanolic extract at 500 mg/ml conc. with a 21.1 mm zone of inhibition. For methanolic extract, at 500 mg/ml conc. Bacillus cereus and Methicillin-resistant Staphylococcus aureus were found more sensitive with 15.4 mm and 15.1 mm ZOI, respectively. Streptococcus pneumoniae was resistant to methanolic Neem extract. Three Gram negative bacteria: Klebsiella pneumoniae, Escherichia coli, and Salmonella typhimurium were resistant to all Neem leaves extracts. The best antimicrobial results of ethanolic and methanolic extracts against Pseudomonas aeruginosa were at 200 mg/ml conc. of each extract with 18.3 and 21.2 mm ZOI, respectively. Negative results were found for aqueous extract with all tested pathogenic bacteria. HPLC analysis of Neem leaves extracts depicted that benzoic acid was detected in ethanolic extract, while ferulic acid and 4-hydroxy benzoic acid were found in all three Neem leaf extracts. This study confirmed the antibacterial activity of Neem leaves against these bacterial strains and the possibility of employing this knowledge in new antibacterial drug development.


How to cite this article
Vancouver
Almaghamsi AW, Gull M, Jambi E, Al-Hejin A, Bataweel NM, Qattan SYA. Antimicrobial Potential of Azadirachta indica (Neem) Leaves Extracts Against Various Pathogenic Bacteria. J Biochem Technol. 2023;14(2):117-24. https://doi.org/10.51847/mow6WkVzIg
APA
Almaghamsi, A. W., Gull, M., Jambi, E., Al-Hejin, A., Bataweel, N. M., & Qattan, S. Y. A. (2023). Antimicrobial Potential of Azadirachta indica (Neem) Leaves Extracts Against Various Pathogenic Bacteria. Journal of Biochemical Technology, 14(2), 117-124. https://doi.org/10.51847/mow6WkVzIg
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JOURNAL OF BIOCHEMICAL TECHNOLOGY
JOURNAL OF BIOCHEMICAL TECHNOLOGY
Journal of Biochemical Technology is a double-blind peer reviewed International Journal published by the Sevas Publishing on behalf of the Biochemical Technology Society, a Registered Charity Organization from India

AREA OF INTEREST
AREA OF INTEREST
new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.

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FOCUS AND SCOPE
Journal Of Biochemical Technology Provides A Medium For The Rapid Publication Of Full-Length Articles, Mini-Reviews Of New And Emerging Products And Short Communications On All Aspects Of ...

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