Diesel, a refinery product of crude oil constitutes a major source of pollution in our environment. Poor solubility in water and the higher content in sediments make diesel a potential water pollutant. Bioremediation of contaminated aquatic and soil environments has arisen as an effective technology, with a range of advantages compared to more traditional methods. A total of 9 bacteria were isolated from three petroleum contaminated soil samples and isolate 3 of sample 1 showed maximum degradati
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Peptide ABMOK1 is composed of 12 amino acid and can interact with the membrane of Escherichia coli. We have carried out isolation, sequencing, prediction, and synthesis of ABMOK1, which is a peptide of membrane activity. LC-MS/MS mapped the ABMOK1 primary structure, CGYCGACVGVCK; SOPMA predicted secondary structure and random coil, while CAMP was used to predict the antimicrobial probability. LC-MS/MS, SOPMA, and CAMP from this study provide the foundation for comprehensive functional and struct
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Abstract The hydrogen production in the cyanobacterium Synechocystis sp. strain PCC6803-GT in the dark anaerobic nitrate free buffer solution with or without monosaccharide has been studied by following the time courses of the amounts per culture volume of hydrogen, exogenous monosaccharide, endogenous glucose in glycogen, dry weight biomass, acetate, lactate and succinate. The monosaccharides utilized in this work were reducing sugars, which were
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Bromelain was coupled to N-isopropyl acrylamide (NIPAm)polymers, synthesized using NIPAm and various concentrations of acrylamide (Ac) or acrylic acid (AAc). Incorporation of Ac/AAcinto the polymer increased the LCST (lower critical solutiontemperature) in a concentration dependent manner but AAc wasmore effective in this regard; the LCST rose to 40°C when 6 percent AAc was used. Incorporation of Ac/AAc increased the coupling of enzyme to the polymer and the η (effectiveness factor) of t
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Mycophenolic Acid (MPA) is an immunosuppressant; produced by Penicillium Sp. In present study an analytical method is developed for analysis of MPA in fermentation broth by High Performance Thin Layer Chromatography (HPTLC), which has been a routine analytical technique by virtue of its advantages of low operating cost, high sample throughput and need for minimum sample clean-up. The method was carried out in TLC precoated silica gel on aluminium plate 60 F254. Various mobile phases were s
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Peripheral blood mononuclear cells (PBMC) comprise of lymphocytes and monocytes which are involved in the pathogenesis of chronic inflammatory diseases. A method for estimation of PBMC lipid composition was modified from an existing method for plasma to support our fish oil intervention study in mild-moderate asthmatics. This modification involved an hour- long direct transesterification reaction using methanol and acetyl chloride followed by a modified GC-FID method. The identification of PBMC
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