Issue 2 Volume 5 (2014)

Heterologous expression and in-silico characterization of Pathogenesis related protein1 (CsPR1) gene from Camellia sinensis.
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Pathogenesis related protein1 gene induced after pathogen infection in plantshave been frequently used as marker gene for systemic acquired resistance. We have carried out isolation, annotation and expression of CsPR1, a potential disease resistance gene. The full length cDNA consist of 671 bp in length containing 162 amino acids with a signal peptide of 22 amino acids and 17.92 kDa predicted molecular weight. Recombinant CsPR1 was successfully expressed in BL21(DE3)pLysS cells using pET 43.1 EK
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Crude fatty acid extracts of Streptomyces sps inhibits the biofilm forming Streptococcus pyogenes ATCC 19615
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Crude fatty acid extract of soil Streptomyces sps on the biofilm formation by Streptococcus pyogenes ATCC 19615 was investigated. Totally, 25 Streptomyces sps were isolated identified from the soil samples collected from Nilgiris hill station. All the isolates were subjected to hydrogen peroxide assay, fatty acid extraction and antibiofilm assay. The fatty acid extracts of S8, S9, and S15 inhibited S. pyogenes at MIC 10 µg/ml. The BIC was observed as 84.6% , 96.41%, 80.5% at 50 µg/ml
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Protoplast fusion technology for improved production of coenzyme Q10 using Paracoccus denitrificans ATCC 19367 mutant strains
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Induced mutants generated from Paracoccus denitrificans ATCC 19367 having antibiotic resistant markers, were used as parent strains to carry out protoplast fusion. The generated fusants were screened using standardized protocol for CoQ10 production. Among the generated fusants, one fusant namely PF-P1 showed 1.73 folds enhancements in specific CoQ10 content than wild type strain. Fusant PF-P1 was characterized by biochemical and molecular approaches where it showed differences than wild type str
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Isolation and characterization of Psedomonas Syringae for nitrate removal under aerobic conditions
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This study describes the isolation and characterization of a novel bacterium for denitrification process under aerobic conditions.  Twenty two denitrifying strains were isolated from sludge of secondary clarifier of waste water treatment plant of a local fertilizer industry.  The isolated strains were initially screened using solid media for their denitrifying activities. Among them one of the bacterium displayed the highest reduction of nitrate without much accumulation of nitrite. Th
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Scale up production of Protease using Pseudomonas aeruginosa MCM B-327 and its Detergent Compatibility
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The maximum protease activity was obtained from P. aeruginosa MCM B-327 with soybean meal 1%, tryptone 1%, initial medium pH 7, agitation rate 250 rpm, aeration rate 0.75 vvm and fermentation temperature 30 °C, under submerged fermentation conditions (SmF). The protease productivity at 10 and 120L fermenters was found to be 16,021 and 9,975 UL-1h-1 respectively. Kinetics of cell growth revealed that specific cell growth rate was 0.025 h-1. Protease was active and stable at differen
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Possible Molecular Targets of Cinnamon in the Insulin Signaling Pathway
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Cinnamon (CN) is known for its anti-diabetic activities in traditional medicine. CN extracts are reported to have beneficial effects on normal and impaired glucose tolerance, insulin resistance and type-2 diabetes. However, molecular characterization of cinnamon effects is limited. The aim of this study is to observe the effect of CN extract on certain diabetogenes involved in insulin signaling. Streptozotocin (STZ) induced type-2 diabetic rats were given CN extract for one month and its effect
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Pleurotus ostreatus: an oyster mushroom with nutritional and medicinal properties
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Mushrooms constitute an integral part of the normal human diet and in recent times, the amounts of consumption have been raised greatly, which includes variety of species. The genus Pleurotus comprise about 40 different species that are commonly referred to as “Oyster mushroom”. Among several species of this genus, Pleurotus ostreatus (P. ostreatus) is popularly consumed by all over the world due to their taste, flavor, high nutritional values and medicinal properties. Because of the
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JOURNAL OF BIOCHEMICAL TECHNOLOGY
JOURNAL OF BIOCHEMICAL TECHNOLOGY
Journal of Biochemical Technology is a double-blind peer reviewed International Journal published by the Deniz Publication on behalf of the Biochemical Technology Society, a Registered Charity Organization from India

AREA OF INTEREST
AREA OF INTEREST
new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.

FOCUS AND SCOPE
FOCUS AND SCOPE
Journal of Biochemical Technology provides a publication on all aspects of biochemistry, biotechnology & bioinformatics and applications in biology and medicine. Areas of high interest cover new advances in enzymatic and protein mechanisms; applied molecular genetics and biotechnology; computational biology, genomics and proteomics; metabolic & tissue engineering; medical, environmental, Pharmacy and pharmaceutical chemistry, food and agro-biotechnology.

Issue 3 Volume 15 - 2024