Statistically based experimental designs were applied for endopolygalacturonase production by mutated strains of Aspergillus niger using apple pomace waste in solid-state fermentation (SSF). The Plackett-Burman design was used to search for the main factors. Urea was found to be the most significant factor (p<0.05) among these variables. To study the mutual interactions between variables and find the optimum medium, Doehlert design was performed to investigate the effect of the medium compone
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α-Amylase and β-glucosidase are key enzymes involved in carbohydrates breakdown and intestinal absorption, respectively. Inhibition of these enzymes hinders blood glucose level increase after a carbohydrate diet and can be an important strategy in the management of non-insulin-dependent diabetes mellitus (NIDDM). A main drawback of currently used inhibitors is their side effects such as abdominal distention, flatulence, meteorism and diarrhea,
caused by excessive inhibition of pancreatic α-am
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The prevalence of colon cancer has rapidly risen during the last decade. In this study we have evaluated the chemopreventive efficacy of ginger in 1,2-dimethyl hydrazine (DMH) induced colon carcinogenesis. Rats were given a weekly subcutaneous injection of DMH at a dose of 20mg/kg body weight for 15 weeks. Ginger (50mg/kg body weight/day) was given at the initiation and also at the post-initiation stages of carcinogenesis to DMH treated rats every day. The animals were sacrificed at the end of 3
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Nine clinical Candida strains (Candida dublinienses (one), Candida albicans (six), and C. tropicalis (two)) were characterized
by production of secreted protease, activity of endoproteinase A and with the HiCandida identification kit. Protease secretion was in
the range of 1.3-1.8 cm while PrA activity was between 0.19 and 0.44 μg/min/mg protein. Results are indicative of the possible use of
endoproteinase activity and SAP production for characterizing Candida strains. Keywords:Biochemical acti
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Journal of Biochemical Technology is a double-blind peer reviewed International Journal published by the Deniz Publication on behalf of the Biochemical Technology Society, a Registered Charity Organization from India
new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.
Journal of Biochemical Technology provides a publication on all aspects of biochemistry, biotechnology & bioinformatics and applications in biology and medicine. Areas of high interest cover new advances in enzymatic and protein mechanisms; applied molecular genetics and biotechnology; computational biology, genomics and proteomics; metabolic & tissue engineering; medical, environmental, Pharmacy and pharmaceutical chemistry, food and agro-biotechnology.