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2012 Volume 3 Issue 4

Study of serum aggrecan fragments in handigodu disease precipitated by DMB dye


Abstract
Handigodu disease is a disorder of osteoarticular system, specific to particular region viz shimoga and chickmagalore districts of Karnataka state, India. The present study focuses to understand the metabolism of aggrecan, a key glycoprotein of cartilage matrix in Handigodu Disease (HD) using serum specimen. The Dimethylmethylene Blue Chloride (DMB) dye was used to precipitate glycosaminoglycans (GAGs) associated proteins in serum. DMB dye was removed from GAGs associated proteins and electrophoresed on SDS-PAGE. The five GAGs associated protein bands were separated, among them two bands; 21.5 KDa and 55.4 KDa were immunoreactive to anti-human aggrecan antibody. These two GAGs associated protein bands were more dense (86% for 21.5 KDa and 61 % for 55.4 KDa) in affected group when compared to unaffected group in Handigodu Disease. These results may imply defective metabolism of aggrecan in a disease condition. The study needs to be extend for other arthritis / dysplastic bone disorders.

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JOURNAL OF BIOCHEMICAL TECHNOLOGY
JOURNAL OF BIOCHEMICAL TECHNOLOGY
Journal of Biochemical Technology is a double-blind peer reviewed International Journal published by the Deniz Publication on behalf of the Biochemical Technology Society, a Registered Charity Organization from India

AREA OF INTEREST
AREA OF INTEREST
new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.

FOCUS AND SCOPE
FOCUS AND SCOPE
Journal Of Biochemical Technology Provides A Medium For The Rapid Publication Of Full-Length Articles, Mini-Reviews Of New And Emerging Products And Short Communications On All Aspects Of ...

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This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. Keywords include, Biochemical Research: Endo/exocytosis, Trafficking, Membrane Biology, Cell Migration, Cell-Matrix Organelle Biogenesis, Cytoskeleton Proteolysis, Cell Death, Cell Cycle, Cancer, Cell Growth/Death, Differentiation, Drug Targets, Gene Therapy, Models of Disease, Proteomics, Stem Cells, Bioenergetics, Mitochondria, Free Radicals, Redox Signaling, Ion Transport/Channels, Oxidative