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2013 Volume 5 Issue 1

Specific activity of glycosidases in brain tumors and their expression in primary explants culture


Abstract
Glycosidases such as β-galactosidase, β-glucosidase and β-hexosaminidase were assayed by monitoring the release of product methyl umbelliferone spectro-flourimetrically in meningiomas (n=96), gliomas (n=101) and in their derived cell lines (n=17) and (n=10) respectively. Glycosidases showed significant specific activity in case of brain tumors as compared to normal brain. β-galactosidase and β-Hexosaminidase has got more activity in all meningiomas and in majority of gliomas, while passages expressed more activity (in first) in majority of meningiomas and gliomas. However β-hexosaminidase  showed higher activity in respective passages of all glioma cell cultures when compared with parent tumors, but not for β-glucosidase. Activity of β-glucosidase did not show significant changes in tumors to that of normal brain. Therefore β-galactosidase and β-hexosaminidase exhibited similar activity in brain tumors and in their cell cultures which confirms their identical enzymatic function in metabolism of tumors related to the stability in confluency of monolayer cells of primary explants.

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JOURNAL OF BIOCHEMICAL TECHNOLOGY
JOURNAL OF BIOCHEMICAL TECHNOLOGY
Journal of Biochemical Technology is a peer reviewed International Journal published by the Sevas Publishing on behalf of the Biochemical Technology Society, a Registered Charity Organization from India

AREA OF INTEREST
AREA OF INTEREST
Areas of high interest, cover new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.

FOCUS AND SCOPE
FOCUS AND SCOPE
Journal Of Biochemical Technology” Provides A Medium For The Rapid Publication Of Full-Length Articles, Mini-Reviews Of New And Emerging Products And Short Communications On All Aspects Of ...

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This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. Keywords include, Biochemical Research: Endo/exocytosis, Trafficking, Membrane Biology, Cell Migration, Cell-Matrix Organelle Biogenesis, Cytoskeleton Proteolysis, Cell Death, Cell Cycle, Cancer, Cell Growth/Death, Differentiation, Drug Targets, Gene Therapy, Models of Disease, Proteomics, Stem Cells, Bioenergetics, Mitochondria, Free Radicals, Redox Signaling, Ion Transport/Channels, Oxidative