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2010 Volume 2 Issue 4

Screening of Streptomyces sp. MTCC4 for the Production of Antifungal Compound against Sclerotium rolfsii


Abstract
Sclerotium rolfsii is a pathogenic fungus, infects about 500 plant species of about 100 families. Streptomyces species MTCC4 show remarkable activity against this fungus. The culture broth of Streptomyces species MTCC4  has inhibitory effect on the fungus in petriplate around the paper disc. Crude culture shows peaks at 2.8, 3.5, 3.8, 4.0, 5.4, 5.9and  8.43  min in HPLC  analysis at the 220 nm wavelength at the flow rate of 0.5 ml/min, in C18 column. After extraction and  purification of each fraction, the fraction with retention time 4.1 min at the Flow rate of  0.5ml/min and detection wavelength 310 nm, show maximum  antifungal activity. 50% Methanol is used as mobile phase for HPLC analysis. Carbohydrate and protein test confirm the presence of carbohydrates and proteins. This compound is identified as a glycoprotein of 11.9 KD have antifungal activity against S. rolfsii.

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J BIOCHEM TECH
J BIOCHEM TECH
Journal of Biochemical Technology is a peer reviewed International Journal published by the Sevas Publishing on behalf of the Biochemical Technology Society, a Registered Charity Organization from India

AREA OF INTEREST
AREA OF INTEREST
Areas of high interest, cover new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.

FOCUS AND SCOPE
FOCUS AND SCOPE
Journal Of Biochemical Technology” Provides A Medium For The Rapid Publication Of Full-Length Articles, Mini-Reviews Of New And Emerging Products And Short Communications On All Aspects Of ...

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This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. Keywords include, Biochemical Research: Endo/exocytosis, Trafficking, Membrane Biology, Cell Migration, Cell-Matrix Organelle Biogenesis, Cytoskeleton Proteolysis, Cell Death, Cell Cycle, Cancer, Cell Growth/Death, Differentiation, Drug Targets, Gene Therapy, Models of Disease, Proteomics, Stem Cells, Bioenergetics, Mitochondria, Free Radicals, Redox Signaling, Ion Transport/Channels, Oxidative