Recent progress in refolding of the C terminal domain of recombinant human alpha-fetoprotein


The C-terminal domain of human alpha-fetoprotein (AFP‑3D) is a fragment that can be used as a vector for targeted delivery of drugs into tumor cells. A technique of recombinant human AFP‑3D refolding in the mobile phase of a gel‑filtration medium was developed and shown to produce a 90% yield of the target protein with confirmed functional activity.