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2020 Volume 11 Issue 3

Process Variable Optimization for the Liquefaction of Guava Fruit Pulp using Soluble Enzyme by Response Surface Methodology


M. Mukunda Vani, R. Satish Babu, M. B. Venkata Ramana Reddy
Abstract

Horticultural fruits are seasonal and are highly perishable with very low-shelf life. Liquefaction of fruits is one such effective technique. In the vegetable and fruit processing industry, pectinase enzymes are used to separate and clarify the juice. Process variables namely enzyme concentration, time, temperature, and pH affect enzymatic liquefaction of guava fruit pulp. These variables can be studied independently. However, all the variables can be studied at a time and also optimized using the statistical tool Response Surface Methodology (RSM) which is adopted here. The optimum conditions for the liquefaction process using soluble enzyme by RSM were- enzyme concentration (0.121% v/v), time (95 min), Temperature (32.7 0C), and pH (2.758). At these variables, the maximum yield was 79.7% and the clarity and viscosity were 0.0475 abs and 486.66 cP respectively.


How to cite this article
Vani M M, Babu R S, Ramana Reddy M B V. Process Variable Optimization for the Liquefaction of Guava Fruit Pulp using Soluble Enzyme by Response Surface Methodology. J Biochem Technol 2020;11(3):68-76
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J BIOCHEM TECH
J BIOCHEM TECH
Journal of Biochemical Technology is a peer reviewed International Journal published by the Sevas Publishing on behalf of the Biochemical Technology Society, a Registered Charity Organization from India

AREA OF INTEREST
AREA OF INTEREST
Areas of high interest, cover new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.

FOCUS AND SCOPE
FOCUS AND SCOPE
Journal Of Biochemical Technology” Provides A Medium For The Rapid Publication Of Full-Length Articles, Mini-Reviews Of New And Emerging Products And Short Communications On All Aspects Of ...

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This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. Keywords include, Biochemical Research: Endo/exocytosis, Trafficking, Membrane Biology, Cell Migration, Cell-Matrix Organelle Biogenesis, Cytoskeleton Proteolysis, Cell Death, Cell Cycle, Cancer, Cell Growth/Death, Differentiation, Drug Targets, Gene Therapy, Models of Disease, Proteomics, Stem Cells, Bioenergetics, Mitochondria, Free Radicals, Redox Signaling, Ion Transport/Channels, Oxidative