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2015 Volume 6 Issue 2

Optimized lactic acid production from whey using hybrid design and ridge analysis


Abstract
Cheese whey, a byproduct of dairy industry, is usually considered as a high strength wastewater from environmental point of view because of its high chemical oxygen demand. Production of valuable chemicals from cheese whey has been considered as an attractive option because of its rich nutrient content. This investigation aimed at the optimized production of lactic acid from cheese whey using isolated Lactobacillus plantarum JX183220 under submerged fermentation by applying response surface methodology (RSM) that involved 4-variable hybrid design and ridge analysis. The effect of different process variables such as whey concentration, yeast extract concentration, inoculum size, and pH of the medium were monitored so as to enhance the lactose conversion in whey to lactic acid. By applying 416C version of hybrid design along with ridge analysis, the maximum production of lactic acid was found to be 36.09 g/L at the optimum combinations of process variables: whey concentration (% v/v) 76.96, yeast extract concentration (% w/v) 1.163, inoculum percentage (% v/v) 4.47 , and pH 6.8.

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J BIOCHEM TECH
J BIOCHEM TECH
Journal of Biochemical Technology is a peer reviewed International Journal published by the Sevas Publishing on behalf of the Biochemical Technology Society, a Registered Charity Organization from India

AREA OF INTEREST
AREA OF INTEREST
Areas of high interest, cover new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.

FOCUS AND SCOPE
FOCUS AND SCOPE
Journal Of Biochemical Technology” Provides A Medium For The Rapid Publication Of Full-Length Articles, Mini-Reviews Of New And Emerging Products And Short Communications On All Aspects Of ...

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This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. Keywords include, Biochemical Research: Endo/exocytosis, Trafficking, Membrane Biology, Cell Migration, Cell-Matrix Organelle Biogenesis, Cytoskeleton Proteolysis, Cell Death, Cell Cycle, Cancer, Cell Growth/Death, Differentiation, Drug Targets, Gene Therapy, Models of Disease, Proteomics, Stem Cells, Bioenergetics, Mitochondria, Free Radicals, Redox Signaling, Ion Transport/Channels, Oxidative