Quick access

Full HTML PDF
Share Article:
2020 Volume 11 Issue 2

Nutritional Value, Cytotoxic and Antimicrobial Activities of Stevia rebaudiana Leaf Extracts


Eman S. Ibrahem, Eman M. Ragheb, Fatimah M. Yousef, Mahmoud F. Abdel-Azizand, Budour A. Alghamdi
Abstract

Stevia rebaudianaBertoni (Stevia), is a plant species in the genus Stevia. This study aimed to explore the main active constituents of dried Stevia leaves. Besides, the antimicrobial and cytotoxic properties of 6 Stevia extracts (aqueous, methanol, ethanol, acetone, chloroform, and petroleum ether) were assessed. Stevia leaves were dried by solar energy then the proximal chemical analysis and the total antioxidant capacitywere conducted. The antimicrobial effects of the 6 different solvent extracts of Stevia leaves were assessed against 7 different pathogens using agar well diffusionmethod. Furthermore, the cytotoxic effect of the 6 Stevia extracts was assessed against human breast carcinoma cell line MCF7. Stevia leaves have good nutritional value as well as have a good amount of total antioxidant capacity, total phenols and total flavonoids (20.788mg AAE/g, 29.5 mg GAE/g and 7.105 mg QE/g, respectively). All Stevia extracts decreased the growth of MCF7 cells. The IC50 of acetone, chloroform, water, ethanol, petroleum ether, and methanol were 150, 100, 374, 180, 79, and 228 µg/ml, respectively. The petroleum ether Stevia leaves extract exerted the most potent cytotoxic activity against MCF7. The aqueous extract of Stevia leaves was the most effective against Bacillus cereus, Salmonella typhi, Pseudomonasaeruginosa, Listeria monocytogenes, Escherichiacoli, and Bacillus cereus. The methanolic extract was the most effective extract against Staphylococcus aurous andAspergillusflavus. In conclusion, Stevia leaves contain considerable amounts of several macro and micronutrients. The aqueous extract of Stevia leaves has a significant potential antimicrobial action. Besides, the petroleum ether extract of Stevia leaves was the most potent cytotoxic extract against MCF7 cancer cells.


How to cite this article
Ibrahem E S, Ragheb E M, Yousef F M, Abdel-Azizand M F, Alghamdi B A. Nutritional Value, Cytotoxic and Antimicrobial Activities of Stevia rebaudiana Leaf Extracts . J Biochem Technol 2020;11(2):108-15
INDEXING
ESCI, SCIRUS, BiologyBrowser, Chemical Abstracts, CABI, Intute catalogue, Science Central, EBSCOhost databases, Google Scholars, Genamics JournalSeek, Open J gate, Ulrich's, ResearchGate, Academic Journals Database, CASSI, ACS (American Chemical Society), CiteFactor, and many other international scientific databases.

JOURNAL OF BIOCHEMICAL TECHNOLOGY
JOURNAL OF BIOCHEMICAL TECHNOLOGY
Journal of Biochemical Technology is a peer reviewed International Journal published by the Sevas Publishing on behalf of the Biochemical Technology Society, a Registered Charity Organization from India

AREA OF INTEREST
AREA OF INTEREST
Areas of high interest, cover new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.

FOCUS AND SCOPE
FOCUS AND SCOPE
Journal Of Biochemical Technology” Provides A Medium For The Rapid Publication Of Full-Length Articles, Mini-Reviews Of New And Emerging Products And Short Communications On All Aspects Of ...

Publish with us

Call for papers

Issue 1 Volume 12 - 2021

Publishing steps

1.Prepare
your paper
2.Submit
and revise
3.Track
your research
4.Share
and promote
This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. Keywords include, Biochemical Research: Endo/exocytosis, Trafficking, Membrane Biology, Cell Migration, Cell-Matrix Organelle Biogenesis, Cytoskeleton Proteolysis, Cell Death, Cell Cycle, Cancer, Cell Growth/Death, Differentiation, Drug Targets, Gene Therapy, Models of Disease, Proteomics, Stem Cells, Bioenergetics, Mitochondria, Free Radicals, Redox Signaling, Ion Transport/Channels, Oxidative