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2018 Volume 9 Issue 2

Isolation and identification of Exopolysaccharide producing Cronobacter species from root nodules of leguminous plants


Gitanjali G. Mane, Venkat S. Hamde
Abstract
Exopolysaccharides have various applications due to their unique rheological properties as in food, pharmaceutical and other industries. Exopolysaccharides also have some medical applications as anti-cancer, anti-viral and immune-modulator. In present study, 72 bacterial strains were isolated from root nodules of eight different leguminous plants and screened for exopolysaccharide production. Out of 72 isolates, 15 bacterial isolates showing good growth and exopolysaccharide production in primary screening were secondarily screened. Two isolates of YC7 and YC10 showing maximum exopolysaccharide production of 3.6g/L and 3.0g/L per dry weight, and 890µg/ml of exopolysaccharide were estimated by phenol sulphuric acid assay. Both strains identified by 16s rRNA YC7 were Cronobacter dublinensis subsp. dublinensis DES187(T), and YC10 was Cronobacter muytjensii ATCC 51329(T). The synthetic carbon sources were replaced with agro-industrial waste products. Cane molasses gave maximum exopolysaccharide production (4.25 g/l and 710µg/ml) at 5%, and rice bran showed a negligible effect. The effect of UV mutagenesis was examined (4.35 g/l and 630µg/ml), and showed positive effect on exopolysaccharide production.

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J BIOCHEM TECH
J BIOCHEM TECH
Journal of Biochemical Technology is a peer reviewed International Journal published by the Sevas Publishing on behalf of the Biochemical Technology Society, a Registered Charity Organization from India

AREA OF INTEREST
AREA OF INTEREST
Areas of high interest, cover new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.

FOCUS AND SCOPE
FOCUS AND SCOPE
Journal Of Biochemical Technology” Provides A Medium For The Rapid Publication Of Full-Length Articles, Mini-Reviews Of New And Emerging Products And Short Communications On All Aspects Of ...

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This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. Keywords include, Biochemical Research: Endo/exocytosis, Trafficking, Membrane Biology, Cell Migration, Cell-Matrix Organelle Biogenesis, Cytoskeleton Proteolysis, Cell Death, Cell Cycle, Cancer, Cell Growth/Death, Differentiation, Drug Targets, Gene Therapy, Models of Disease, Proteomics, Stem Cells, Bioenergetics, Mitochondria, Free Radicals, Redox Signaling, Ion Transport/Channels, Oxidative