Introduction: Occupational burnout is a psychological syndrome consisting of emotional exhaustion, depersonalization, and reduced personal accomplishment. This study aimed to investigate burnout among health workers and to determine its related factors in Jahrom University of Medical Sciences. Materials and Methods: This study cross-sectional analysis was conducted in 2018 and the study population consisted of all eligible health workers (Behvarzan) of Jahrom University of Medical Sciences. Data were collected using Maslach Job Burnout Questionnaire and the validity and reliability of the questionnaire had been confirmed. Data were analyzed using SPSS 18, and by ANOVA, Pearson correlation coefficients and independent t-tests. Results: The prevalence of burnout was 56.3% in reduced personal accomplishment, 16.2% in emotional exhaustion and 1.2% in depersonalization. A significant relation was observed between reduced personal accomplishment and satisfaction with income (p = 0.04), age (p = 0.02), work experience (p = 0.002) and level of education (P = 0.001). There were significant relations between emotional exhaustion with interest in job (P =0.04) and satisfaction from authorities (P < 0.0001). Conclusion: Reduced personal accomplishment was prevalent among the participating health workers. It is necessary to apply appropriate interventions for health workers who are dissatisfied with their job and income. Also, it is recommended to consider offering awards, job promotion and organizing workshops to prevent stress among health workers.