Aim: The aim of the study was to evaluate the levels of Interleukin IL-1β, TNFα, and VDB Protein in the serum of patients with chronic periodontitis. Materials and methods: Serum samples of 60 persons, aged 25-55 years with chronic periodontitis were collected as patients group; samples of serum were obtained from 30 healthy subjects aged 25-55 years as the control group. Interleukin IL-1β, TNF-α, and VDB protein levels were measured by enzyme-linked immune sorbent assay (ELISA) kit. Results: The levels of Interleukin IL-1β, TNF-α, and VDB protein in serum showed a significant increase in the patient group than the healthy group. On the other hand, VDB protein was not significantly correlated with IL-1β and TNF-α in patients.