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2008 Volume 1 Issue 2

Effects of leucine-enkephalin on alpha-naphthyl acetate esterase and myeloperoxidase activity in haemolymph of the scallop Chlamys farreri


Abstract
The study was designed to investigate the effects of leucineenkephalin (L-ENK) on the activity of alpha-naphthyl acetate esterase (ANAE) and myeloperoxidase (MPO) in haemolymph of the scallop Chlamys farreri. Our results indicate that MPO activity with DAB and DEA substrate solution was induced when the concentration of L-ENK was 5 and 50 μg/ml, and the intracellular and extracellular MPO activity with two substrate solutions was highest when the concentration of L-ENK was 50 μg/ml. However, both the intracellular and extracellular MPO activity of 1 μg/ml group was significantly lower than that of control group. It was also found that the intracellular and extracellular ANAE activity was induced by L-ENK. The supernatant ANAE activity was highest when the concentration of L-ENK was 50 μg/ml, but the resultant HLS ANAE activity was highest when the concentration of L-ENK was 5 μg/ml. Overall, the data suggests an involvement of L-ENK in the regulation of the defense systems of the scallop Chlamys farreri. L-ENK may function as messengers in the bidirectional interaction between the neuroendocrine and the immune systems Keywords: Chlamys farreri; Leucine-enkephalin; Alpha-naphthyl acetate esterase; Myeloperoxidase; Haemolymph Received: 23 September 2008 / Received in revised form: 6 October 2008, Accepted: 7 October 2008 Published online: 18 October 2008

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J BIOCHEM TECH
J BIOCHEM TECH
Journal of Biochemical Technology is a peer reviewed International Journal published by the Sevas Publishing on behalf of the Biochemical Technology Society, a Registered Charity Organization from India

AREA OF INTEREST
AREA OF INTEREST
Areas of high interest, cover new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.

FOCUS AND SCOPE
FOCUS AND SCOPE
Journal Of Biochemical Technology” Provides A Medium For The Rapid Publication Of Full-Length Articles, Mini-Reviews Of New And Emerging Products And Short Communications On All Aspects Of ...

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This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. Keywords include, Biochemical Research: Endo/exocytosis, Trafficking, Membrane Biology, Cell Migration, Cell-Matrix Organelle Biogenesis, Cytoskeleton Proteolysis, Cell Death, Cell Cycle, Cancer, Cell Growth/Death, Differentiation, Drug Targets, Gene Therapy, Models of Disease, Proteomics, Stem Cells, Bioenergetics, Mitochondria, Free Radicals, Redox Signaling, Ion Transport/Channels, Oxidative