An operational and eco-friendly technique was applied for the synthesis of silver nanoparticles (AgNPs) utilizing Arachis pintoi (A. pintoi) extract as a reducing agent. The features of synthesized AgNPs were investigated utilizing XRD, SEM, TEM, and FT-IR. The formation of AgNPs was verified by UV-Vis spectroscopy at the wavelength range of 400-450 nm. The proper conditions for AgNPs synthesis were determined such as the AgNO3 concentration of 1.75 mM, the volume ratio of AgNO3 solution/A. pintoi extract of 4.0/1.0, stirring rate of 300 rpm, and the synthesis duration of 90 minutes. X-ray diffraction demonstrated a face-centered cubic structure of AgNPs with highly crystalline nature. FT-IR confirmed that flavonoids, phenolic acids, and alkaloid molecules can be bound to AgNP acted both as the reducing and stabilizing agents. SEM and TEM images revealed that obtained AgNP was nearly spherical in shape and uniform in size distribution with the average size of 11.4 nm. AgNPs showed effective antibacterial activity against Staphylococcus aureus with the average inhibition zone diameters of 14 mm and the MIC value of 18.9 µg/mL. Furthermore, its antibacterial activity on the mouse pad was tested against S. aureus.
