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2019 Volume 10 Issue 4

Biosynthesis of silver nanoparticles using Arachis Pintoi extract and its antibacterial activity


Nguyen Phung Anh, Phan Ngo Minh Quang, Duong Trong Van, Luong Thi Cam Van, Duong Nhat Linh, Nguyen Van Minh, Vuong Phu Tai, Nguyen Tri
Abstract

An operational and eco-friendly technique was applied for the synthesis of silver nanoparticles (AgNPs) utilizing Arachis pintoi (A. pintoi) extract as a reducing agent. The features of synthesized AgNPs were investigated utilizing XRD, SEM, TEM, and FT-IR. The formation of AgNPs was verified by UV-Vis spectroscopy at the wavelength range of 400-450 nm. The proper conditions for AgNPs synthesis were determined such as the AgNO3 concentration of 1.75 mM, the volume ratio of AgNO3 solution/A. pintoi extract of 4.0/1.0, stirring rate of 300 rpm, and the synthesis duration of 90 minutes. X-ray diffraction demonstrated a face-centered cubic structure of AgNPs with highly crystalline nature. FT-IR confirmed that flavonoids, phenolic acids, and alkaloid molecules can be bound to AgNP acted both as the reducing and stabilizing agents. SEM and TEM images revealed that obtained AgNP was nearly spherical in shape and uniform in size distribution with the average size of 11.4 nm. AgNPs showed effective antibacterial activity against Staphylococcus aureus with the average inhibition zone diameters of 14 mm and the MIC value of 18.9 µg/mL. Furthermore, its antibacterial activity on the mouse pad was tested against S. aureus.


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J BIOCHEM TECH
J BIOCHEM TECH
Journal of Biochemical Technology is a peer reviewed International Journal published by the Sevas Publishing on behalf of the Biochemical Technology Society, a Registered Charity Organization from India

AREA OF INTEREST
AREA OF INTEREST
Areas of high interest, cover new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.

FOCUS AND SCOPE
FOCUS AND SCOPE
Journal Of Biochemical Technology” Provides A Medium For The Rapid Publication Of Full-Length Articles, Mini-Reviews Of New And Emerging Products And Short Communications On All Aspects Of ...

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This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. Keywords include, Biochemical Research: Endo/exocytosis, Trafficking, Membrane Biology, Cell Migration, Cell-Matrix Organelle Biogenesis, Cytoskeleton Proteolysis, Cell Death, Cell Cycle, Cancer, Cell Growth/Death, Differentiation, Drug Targets, Gene Therapy, Models of Disease, Proteomics, Stem Cells, Bioenergetics, Mitochondria, Free Radicals, Redox Signaling, Ion Transport/Channels, Oxidative