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2020 Volume 11 Issue 1

Biofabrication of silver nanoparticles using Curcuma longa extract: Effects of extraction and synthesis conditions, characteristics, and its antibacterial activity


Nguyen D. Trung, Nguyen T. Nhuan, Luong T. C. Van, Nguyen V. Minh, Nguyen P. Anh, and Nguyen Tri
Abstract

This investigation was done to synthesize silver nanoparticles (AgNPs) using Curcuma longa (C. longa) extract as a capping, and stabilizing, reducing agent. The impact of C. longa raw material/water ratio, extraction duration and temperature, the volume ratios of AgNO3 solution/C. longa extract, and AgNO3 concentration in the formation of AgNPs were evaluated. The obtained AgNPs were analyzed by UV-Vis spectroscopy, FTIR, X-ray diffraction pattern (XRD), energy-dispersive X-ray spectroscopy (EDX), transmission electron microscopy (TEM), and the size distribution estimated by zeta instrument. The presence of AgNPs exhibited surface plasmon resonance peak approximately at 442 nm. FTIR study demonstrated that hydroxyl functional groups existed in the extract that reduced Ag+ to Ag°. XRD pattern was used to identify the crystalline structure of AgNPs. The morphology and size of the synthesized AgNPs were determined by TEM. The obtained AgNPs with a face-centered cubic structure were nearly spherical in shape and 5‒30 nm in diameter, and the average size of particles was 28.0 nm. The antibacterial activity of AgNPs was investigated against Bacillus cereus (B. cereus), Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), and Salmonella typhi (Salmonella) by disc diffusion method.  The inhibition zone of obtained AgNPs against bacteria varied in a range of 15‒20 mm and the minimum inhibitory concentrations were determined only 2.81 - 11.5 mg.ml-1.


How to cite this article
Trung N D, Nhuan N T, Van L T C, Minh N V, Anh N P, Tri A N. Biofabrication of silver nanoparticles using Curcuma longa extract: Effects of extraction and synthesis conditions, characteristics, and its antibacterial activity. J Biochem Technol 2020;11(1):57-66
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J BIOCHEM TECH
J BIOCHEM TECH
Journal of Biochemical Technology is a peer reviewed International Journal published by the Sevas Publishing on behalf of the Biochemical Technology Society, a Registered Charity Organization from India

AREA OF INTEREST
AREA OF INTEREST
Areas of high interest, cover new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.

FOCUS AND SCOPE
FOCUS AND SCOPE
Journal Of Biochemical Technology” Provides A Medium For The Rapid Publication Of Full-Length Articles, Mini-Reviews Of New And Emerging Products And Short Communications On All Aspects Of ...

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This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. Keywords include, Biochemical Research: Endo/exocytosis, Trafficking, Membrane Biology, Cell Migration, Cell-Matrix Organelle Biogenesis, Cytoskeleton Proteolysis, Cell Death, Cell Cycle, Cancer, Cell Growth/Death, Differentiation, Drug Targets, Gene Therapy, Models of Disease, Proteomics, Stem Cells, Bioenergetics, Mitochondria, Free Radicals, Redox Signaling, Ion Transport/Channels, Oxidative