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2014 Volume 5 Issue 3

A time efficient adaptation of GC-FID method for the analysis of PBMC lipid composition


Abstract
Peripheral blood mononuclear cells (PBMC) comprise of lymphocytes and monocytes which are involved in the pathogenesis of chronic inflammatory diseases. A method for estimation of PBMC lipid composition was modified from an existing method for plasma to support our fish oil intervention study in mild-moderate asthmatics. This modification involved an hour- long direct transesterification reaction using methanol and acetyl chloride followed by a modified GC-FID method. The identification of PBMC fatty acid composition was achieved by relative comparison with a 37 Fatty acid component standard. The validation demonstrated the method to be reproducible and accurate with a within batch and between batch CVs of <10%. The modified method was adapted for PBMC total lipid estimation and was considered suitable to detect changes in PBMC composition following fish oil supplementation. This method is quick, scalable, cheaper and cost effective for large scale population and intervention studies. (145 words)

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J BIOCHEM TECH
J BIOCHEM TECH
Journal of Biochemical Technology is a peer reviewed International Journal published by the Sevas Publishing on behalf of the Biochemical Technology Society, a Registered Charity Organization from India

AREA OF INTEREST
AREA OF INTEREST
Areas of high interest, cover new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.

FOCUS AND SCOPE
FOCUS AND SCOPE
Journal Of Biochemical Technology” Provides A Medium For The Rapid Publication Of Full-Length Articles, Mini-Reviews Of New And Emerging Products And Short Communications On All Aspects Of ...

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This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. Keywords include, Biochemical Research: Endo/exocytosis, Trafficking, Membrane Biology, Cell Migration, Cell-Matrix Organelle Biogenesis, Cytoskeleton Proteolysis, Cell Death, Cell Cycle, Cancer, Cell Growth/Death, Differentiation, Drug Targets, Gene Therapy, Models of Disease, Proteomics, Stem Cells, Bioenergetics, Mitochondria, Free Radicals, Redox Signaling, Ion Transport/Channels, Oxidative