Objectives : The study examined the rate and type of microbial contamination of mobile phones of students, professors, and medical staff of the Faculty of Dentistry of Tabriz University of medical sciences. Materials and Methods: 160 participants were selected among the professors, students and medical staff of the faculty of dentistry. The samples were taken from mobile phones and the dominant hands using a wet sterilized swab, and the bacterial isolation and identification were performed following the cultivation. The results were reported as frequency to examine the type of bacteria in hands and phones and the number of bacteria and frequency of use of the phone were examined using mean and standard deviation and frequency-percentage of the results. Washing hands after using the phone was stated by using the information captured from the questionnaires. Results:Microbial contamination in mobile phones was proven in 97.5% of cases. The type of microorganism cultivated from phones was epidermis in 44% of the cases, staphylococcus aureus in 19.5%, both epidermis and aureus in 25.5% of the cases, and other microbes in 8.8% of the cases. There was contamination in the hands of everyone. The type of microorganism cultured from people's hand was 1.3% epidermis, 5% staphylococcus aureus, and 93.7% both epidermis and aureus. The number of microbes both in mobile phones and the hands of people was directly related to the number of conversations and the number of SMS sent (P<0.05). Examining washing hand after using mobile phones showed that in 78.1% of the cases, people do not wash their hands after conversations. Conclusion: According to the results, in most of the cases, the mobile phones and the hands of teachers, students and medical staff of the Faculty of Dentistry of Tabriz University of Medical Sciences were contaminated.