In this present study, we applied validation techniques like specificity, accuracy, precision and robustness to find the thermophysical properties of biodiesel produced using waste vegetable oil and waste cooking oil collected from local restaurants in Ardabil City, Iran. The validation techniques helped in improving the accuracy of the estimation of thermo-physical properties. The properties investigated in this study includes, density, viscosity, flash point, cloud point and heating value. We believe that this validation technique will have wide applications in biodiesel production.