At present, much attention is paid to various aspects concerning the impact of systematic muscular load on platelet parameters. In our study, we examined 107 healthy mature males. They were divided into 4 groups. In this study, we determined the mean platelet volume, their aggregation activity when introduced into plasma with 2.5, 5, and 10 μM ADP, and the amount of GP IIb-IIIa and GP Ib on the platelet surfaces. With an increase in daily muscle activity, the examined patients showed a weakening in the process of platelet aggregation in relation to all the applied concentrations of ADP, the content of adenosine phosphates in them, and the secretion of adenosine phosphates from them, the platelet level of actin and myosin, a decrease in platelets size and a reduction in the GP IIb-IIIa and GP Ib expressiveness. There was revealed the correlation between the volume of platelets, on the one hand, and platelet aggregation, the adenosine phosphates content in them and secretion from them, the level of actin and myosin self-assembly, and the density of GP IIb-IIIa and GP Ib receptors on platelet membranes, on the other hand, which, to a greater extent, manifested itself in the most physically active people. The changes in platelet parameters that occur in males of the second mature age if the distance traveled per day increases indicate the possibility of their optimization through a rational and controlled increase in physical activity within a day.