Migraine is a common disabling primary headache disorder. It affects 6% of men and 18% of women worldwide and 12.1% in Saudi Arabia. The lack of many nutrients like vitamin B12 and magnesium is associated with migraine. Migraine may be socicated with cardiovascular risk factors such as a high level of cholesterol. Ninety-one Saudi female patients with a mean age of 32.91±1.187 and fifty-four healthy Saudi females (control) with a mean age of 30.00±1.32 years participated in this study. Serum lipid profile, vitamin B12, and magnesium levels were measured in both patients and controls. The results showed a significant increase in serum cholesterol and low-density lipoprotein (LDL), and lower levels of high-density lipoprotein (HDL) for migraine patients. However, no significant difference has been observed in triglyceride serum levels. No significant differences were found in vitamin B12 and magnesium (Mg) serum levels between the two groups (migraine patients and control). In conclusion, lipid profile was shown to be associated with Saudi female patients with migraine. Vitamin B12 serum levels and mg serum levels were not significantly correlated in migraine patientsand controls.