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2020 Volume 11 Issue 1

Synthesis of silver nanoparticles using Aeschynomene indica L. aqueous leaf extract and evaluation of its Antibacterial activity


Kataru Vishnuvardhan, Bommana Kavitha, Yasodamma Nimmanapalli
Abstract

Green nanoparticle synthesis methods have immense therapeutic applications due to their biocompatibility and lack of toxicity. We report the synthesis of silver nanoparticles (AgNPs) using the leaf extracts of traditional medicinal plant Aeschynomene indica (Fabaceae). The color change from yellow to brown and absorption peak at 447 nm indicated the synthesis of AgNPs. High-resolution transmission electron microscopy images exhibit polydisperse, spherical AgNPs in the size range of 10-24nm. X-ray diffraction studies confirmed the crystalline nature with a face-centered cubic (FCC) structure of the AgNPs. The stability of the nanoparticles was confirmed by dynamic light scattering and zeta potential studies. FTIR spectroscopy revealed the presence of compounds with functional groups such as primary amines, phenols, aldehyde/ketone, aromatic, and nitro groups, which may be responsible for the reduction of AgNPs. The antibacterial activity of plant extracts and synthesized AgNPs was confirmed by agar well diffusion method. The synthesized AgNPs showed potential antibacterial activity with a zone of inhibition of 14.5 mm on Pseudomonas aeruginosa; 14.2 mm on Escherichia coli; 11.5 mm on Staphylococcus aureus; and 10.3 mm on Bacillus subtilis. Also, the silver nanoparticles exhibited a 73.9% antioxidant activity confirmed by DPPH analysis. The antibacterial and antioxidant activities of AgNPs synthesized from leaf extracts of A. indica can be employed for biomedical applications.


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JOURNAL OF BIOCHEMICAL TECHNOLOGY
JOURNAL OF BIOCHEMICAL TECHNOLOGY
Journal of Biochemical Technology is a double-blind peer reviewed International Journal published by the Deniz Publication on behalf of the Biochemical Technology Society, a Registered Charity Organization from India

AREA OF INTEREST
AREA OF INTEREST
new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.

FOCUS AND SCOPE
FOCUS AND SCOPE
Journal of Biochemical Technology provides a publication on all aspects of biochemistry, biotechnology & bioinformatics and applications in biology and medicine. Areas of high interest cover new advances in enzymatic and protein mechanisms; applied molecular genetics and biotechnology; computational biology, genomics and proteomics; metabolic & tissue engineering; medical, environmental, Pharmacy and pharmaceutical chemistry, food and agro-biotechnology.

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This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. Keywords include, Biochemical Research: Endo/exocytosis, Trafficking, Membrane Biology, Cell Migration, Cell-Matrix Organelle Biogenesis, Cytoskeleton Proteolysis, Cell Death, Cell Cycle, Cancer, Cell Growth/Death, Differentiation, Drug Targets, Gene Therapy, Models of Disease, Proteomics, Stem Cells, Bioenergetics, Mitochondria, Free Radicals, Redox Signaling, Ion Transport/Channels, Oxidative