Throughout the years, azo compounds including Sudan dyes have been widely used in industry as colorants in food, cosmetics, waxes, solvents, textiles, plastics and printing inks. Sudan dyes I, II, III, IV, and their degradation products have been regarded to hurt human health. They cause cancer due to their teratogenicity, genotoxicity and carcinogenicity effects. In the present work, the efficiency of ferrous sulfate with low cost and the first choice in the treatment of printing, dyeing and electroplating wastewater to reduce the toxicity of Sudan II-induced oxidative damage to hematological parameters, liver and kidney functions in adult female and male rabbits was investigated. Animals which received Sudan II were treated with ferrous sulfate as the sole source of potent liquid for 14 days at the dose of 111mg/kg B.wt before being sacrificed. Hematological parameters (total leukocytes (WRBs), erythrocytes (RBCs) count hemoglobin content (Hb) and hematocrit percentage (Ht%), liver and kidney functions were done to check the efficiency of ferrous sulfate as Sudan II detoxification. The obtained data clearly showed that Sudan II toxicity led to a significant reduction in (WBCs, RBCs, Hb, and Ht) of the hematological parameters. Sudan II led to an elevation in the activity of liver function enzymes (transaminase; ALT and AST), a decrease in Alkaline phosphatase ALP and an increase in kidney functions (urea and creatinine). The detoxification properties of ferrous sulfate were obvious thanks to good flocculation and decolorization, utilized to separate heavy metal ions, oils, phosphorus and forpurification, and wastewater. Finally, the present study proved that Sudan II treated with ferrous sulfate can keep hematological parameters, liver and kidney functions near the physiological normal state.