An eye defense mechanism called the blood-ocular barrier stops hazardous compounds from the circulatory system from entering the eye's structural components. In order to preserve human health and quality of life, the blood-ocular barrier must operate at its peak. There are several eye illnesses that might be linked to a breach in the blood-ocular barrier's functionality. The blood-ocular barrier's permeability to medications in the levomecitin group was examined in this paper under both healthy and unhealthy circumstances. A total of 40 laboratory rabbits were divided into two groups for this reason, and the experimental group's rabbits underwent artificial induction of keratoconjunctivitis. In the posterior femoral muscle group of rabbits in the control and experimental groups, a 30 mg/kg dose of a 10% chloramphenicol solution diluted in a 0.5% novocaine solution was intramuscularly administered twice, separated by a 12-hour interval. Next, the behavior and health of the rabbits in both groups were observed, along with routine checks on the animals' body temperatures, pulses, and respirations. On several experiment days, the level of chloramphenicol in blood serum and intraocular fluid was examined in order to gauge the permeability of the blood-ocular barrier. Further research was done on the biochemical and hematological characteristics of blood serum.