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2018 Volume 9 Issue 3

Simultaneous Estimation of Chlorpromazine hydrochloride and Carvedilol in Bulk and Pharmaceutical Dosage Forms Using HPLC


Sahar Rihan Fadhel, Shaymaa Ibrahim Khalil
Abstract
A simple, rapid and accurate HPLC method was developed and assessed for the simultaneous estimation of Chlorpromazine hydrochloride and Carvedilol in its bulk and Pharmaceutical tablets. The separation was obtained by using C18 ec (250 × 4.6 mm, 5μm) column with a mobile phase composing of acetonitrile: deionized water (50: 50 v/v, pH adjusted to 3.6 ± 0.03 with acetic acid) at flow rate of 1.5 mL.min-1. The detection was carried out at 237nm, the retention time of CLO, and CARs were 1.56 and 3.84 min; respectively. The linearity range of 2-150 μg/mL and 1-150 μg/mL were selected for CLO and CAR; respectively. The correlation coefficients were found to be 0.9995 and 0.9999, and the limits of detection were found to be 0.6259μg/mL and 0.9365μg/mL for CLO and CAR; respectively. All the validation parameters were found to be within the acceptance limit. The developed HPLC method can be successfully used for the quantitative estimation of the two cited drugs in their formulation.

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JOURNAL OF BIOCHEMICAL TECHNOLOGY
JOURNAL OF BIOCHEMICAL TECHNOLOGY
Journal of Biochemical Technology is a double-blind peer reviewed International Journal published by the Sevas Publishing on behalf of the Biochemical Technology Society, a Registered Charity Organization from India

AREA OF INTEREST
AREA OF INTEREST
new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.

FOCUS AND SCOPE
FOCUS AND SCOPE
Journal Of Biochemical Technology Provides A Medium For The Rapid Publication Of Full-Length Articles, Mini-Reviews Of New And Emerging Products And Short Communications On All Aspects Of ...

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This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. Keywords include, Biochemical Research: Endo/exocytosis, Trafficking, Membrane Biology, Cell Migration, Cell-Matrix Organelle Biogenesis, Cytoskeleton Proteolysis, Cell Death, Cell Cycle, Cancer, Cell Growth/Death, Differentiation, Drug Targets, Gene Therapy, Models of Disease, Proteomics, Stem Cells, Bioenergetics, Mitochondria, Free Radicals, Redox Signaling, Ion Transport/Channels, Oxidative