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2011 Volume 3 Issue 2

Seeds of rosary pea, Abrus precatorius : A novel source of hydroxynitrile lyase


Abstract
Seeds of Abrus precatorius (rosary pea) were identified as a new and potential source of hydroxynitrile lyase. Hydroxynitrile lyase from the seeds of this plant was purified up to 9 fold with specific activity of 577 Umg-1 protein by using ion exchange and gel filtration chromatographic techniques. The purified enzyme was a heteromer with estimated native molecular mass 205 kDa and its subunits showed two bands of molecular mass of 42.0 and 36.5 kDa in SDS-PAGE, respectively. The enzyme exhibited maximum activity at 30ºC with 0.1 M sodium citrate buffer (pH 5). It has KM of 13 mM and Vmax of 625 Umg-1 of protein with mandelonitrile as a substrate. In 120 ml of reaction mixture containing 25 ml of benzaldehyde (substrate) and 6.75 mg of purified enzyme produced 17.5 g of mandelonitrile. 

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JOURNAL OF BIOCHEMICAL TECHNOLOGY
JOURNAL OF BIOCHEMICAL TECHNOLOGY
Journal of Biochemical Technology is a double-blind peer reviewed International Journal published by the Deniz Publication on behalf of the Biochemical Technology Society, a Registered Charity Organization from India

AREA OF INTEREST
AREA OF INTEREST
new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.

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FOCUS AND SCOPE
Journal Of Biochemical Technology Provides A Medium For The Rapid Publication Of Full-Length Articles, Mini-Reviews Of New And Emerging Products And Short Communications On All Aspects Of ...

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This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. Keywords include, Biochemical Research: Endo/exocytosis, Trafficking, Membrane Biology, Cell Migration, Cell-Matrix Organelle Biogenesis, Cytoskeleton Proteolysis, Cell Death, Cell Cycle, Cancer, Cell Growth/Death, Differentiation, Drug Targets, Gene Therapy, Models of Disease, Proteomics, Stem Cells, Bioenergetics, Mitochondria, Free Radicals, Redox Signaling, Ion Transport/Channels, Oxidative