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2012 Volume 4 Issue 1

Scale up production of polyhydroxyalkanoate (PHA) at different aeration, agitation and controlled dissolved oxygen levels in fermenter using Halomonas campisalis MCM B-1027


Abstract
Production of biodegradable plastic, polyhydroxyalkanoate (PHA) was demonstrated using culture of moderately haloakalitolerant Halomonas campisalis MCMB-1027. Production of PHA was carried out at different aeration (0.5-1, 1, and 1.5 vvm) and agitation (100,150 and 200 rpm) in 14 L fermenter. Maximum production of PHA was 40.69% on the basis of dry cell mass at 1 vvm and 100 rpm. Material balance over maltose revealed that aeration 1 vvm and agitation 100 rpm was optimum for conversion of utilized maltose into PHA. Controlled dissolved oxygen in the range 1-5% level during PHA accumulation phase facilitated to increase PHA content to 56.23%. A correlation equation was developed by fixing mass transfer coefficient (KLa) and applied successfully for scale up production of PHA in 120 L fermenter. 1H NMR analysis showed percentage of HB and HV unit 95.83 and 4.17 respectively.

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JOURNAL OF BIOCHEMICAL TECHNOLOGY
JOURNAL OF BIOCHEMICAL TECHNOLOGY
Journal of Biochemical Technology is a double-blind peer reviewed International Journal published by the Deniz Publication on behalf of the Biochemical Technology Society, a Registered Charity Organization from India

AREA OF INTEREST
AREA OF INTEREST
new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.

FOCUS AND SCOPE
FOCUS AND SCOPE
Journal of Biochemical Technology provides a publication on all aspects of biochemistry, biotechnology & bioinformatics and applications in biology and medicine. Areas of high interest cover new advances in enzymatic and protein mechanisms; applied molecular genetics and biotechnology; computational biology, genomics and proteomics; metabolic & tissue engineering; medical, environmental, Pharmacy and pharmaceutical chemistry, food and agro-biotechnology.

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This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. Keywords include, Biochemical Research: Endo/exocytosis, Trafficking, Membrane Biology, Cell Migration, Cell-Matrix Organelle Biogenesis, Cytoskeleton Proteolysis, Cell Death, Cell Cycle, Cancer, Cell Growth/Death, Differentiation, Drug Targets, Gene Therapy, Models of Disease, Proteomics, Stem Cells, Bioenergetics, Mitochondria, Free Radicals, Redox Signaling, Ion Transport/Channels, Oxidative