To assess the effect of three months of fitness classes on platelet activity in men. The observation group was 25 clinically healthy men of the first mature age who were engaged in fitness for three months. The control group included 26 men of the first mature age who avoided physical culture and sports during their life. The physical condition was assessed using functional tests and platelet activity was determined by traditional methods. The results were processed by the methods of variation statistics. Regular physical activity in the course of fitness activities increased general physical capabilities in men of the first mature age and increased the accuracy of the movements. This was accompanied by a weakening of platelet aggregation activity, recorded in vivo and in vitro. The onset of physiologically beneficial changes in platelet function is associated with a decrease in the density of receptors on platelets in training men and an increase in the activity of the antioxidant system of platelets. In the control group, the level of physical fitness was significantly lower, and the functional capabilities of platelets were higher. Fitness classes in the first adulthood can optimize the level of platelet activity, transferring it to a more functionally beneficial level for the body. Three-month fitness classes increase the level of physical fitness in men of the first mature age and contribute to a decrease in platelet activity.