In this research, the antimicrobial function of the aqueous and organic essences obtained from Ziziphus jujuba pulp fruits was investigated against strains of bacteria and fungi. The RP-HPLC-PDA analysis was observed in the hydro-acetone (EA) and ethyl acetate extracts (Acet1, Acet2) in the presence of gallic acid, quercetin, and rutin. Most extracts present a slight antimicrobial activity with an inhibition zone not exceeding 6.5 mm diameter, except for S. aureus which was the most sensitive strain. The Minimum Inhibitory Concentration (MIC) values ranged between 450 and 110 mg/mL, E. coli and S. aureus are the most sensitive strains for all extracts. No particular antifungal activity of extracts was observed against C. albicans strain. The antimicrobial activity of Z. jujuba pulp fruits has not been reported before, and the present study has revealed that the phenolic compounds identified in jujube fruit extracts cannot provide an important antimicrobial effect to jujube pulp fruits.