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2012 Volume 3 Issue 4

Optimization of process parameters for tacrolimus (FK 506) production by new isolate of Streptomyces sp. using response surface methodology (RSM)


Abstract
An isolate of Streptomyces was found to be positive for the production of tacrolimus. HPLC analysis on C-18 column at flow rate of 0.5 ml/min samples gave retention time of 4.26 min as standard. Soyoil, soybean meal and L-lysine were selected as significant model factors by plackett burman (PB) design. Response surface methodology was employed to optimize these factors. A second-order quadratic model and response surface method showed that the optimum conditions (soyoil: 23.40 gm/L; soybean meal gm/L: 18.40; L-lysine: 1.00 gm/L) resulted in the improvement of tacrolimus production (235.5± 1.96 mg/L) as compared to the initial level (135.6± 2.56 mg/L). It was about 1.73 fold increase as compared with that using the original medium. The value predicted by the quadratic model was (236.3 mg/L). Analysis of variance showed a high coefficient of determination (R2) value of 0.9978 ensuring a satisfactory adjustment of the quadratic model with the experimental data. This is first report on tacrolimus production using plackett burman design and response surface methodology in submerged fermentation.

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JOURNAL OF BIOCHEMICAL TECHNOLOGY
JOURNAL OF BIOCHEMICAL TECHNOLOGY
Journal of Biochemical Technology is a double-blind peer reviewed International Journal published by the Deniz Publication on behalf of the Biochemical Technology Society, a Registered Charity Organization from India

AREA OF INTEREST
AREA OF INTEREST
new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.

FOCUS AND SCOPE
FOCUS AND SCOPE
Journal Of Biochemical Technology Provides A Medium For The Rapid Publication Of Full-Length Articles, Mini-Reviews Of New And Emerging Products And Short Communications On All Aspects Of ...

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This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. Keywords include, Biochemical Research: Endo/exocytosis, Trafficking, Membrane Biology, Cell Migration, Cell-Matrix Organelle Biogenesis, Cytoskeleton Proteolysis, Cell Death, Cell Cycle, Cancer, Cell Growth/Death, Differentiation, Drug Targets, Gene Therapy, Models of Disease, Proteomics, Stem Cells, Bioenergetics, Mitochondria, Free Radicals, Redox Signaling, Ion Transport/Channels, Oxidative