Optimization of lipase production from an indigenously isolated marine Aspergillus sydowii of Bay of Bengal

A total of 44 marine sediments from 8 locations along the South East Coast of Bay of Bengal were screened for lipolytic fungal isolates by tributyrin agar clearing method and submerged fermentation. Marine fungus BTSS 1005, isolated from Divipoint location was identified as Aspergillus sydowii. Further studies confirmed that BTSS105 produces higher extracellular lipase when compared with other isolates. Efforts to increase the yield were achieved by optimizing the production medium and growth conditions, which would fully exploit the potential of the microorganism. The optimum medium composition at 32°C, 8.0 pH, 80 rpm, 40% volume ratio, 96hr incubation time and 10% (v/v) inoculum, under submerged fermentation was sucrose-2% (w/v); ammonium chloride-3.5% (w/v); olive oil-3% (v/v) and tween 80-0.2% (v/v).