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2021 Volume 12 Issue 1

Molecular Screening for Transfusion Transmissible P. falciparum in Asymptomatic Blood Donors in the Non-endemic Region


Hanaa Mohammed Abd Allah, Emtithal Abdo, Elamin Abd elkareem Elamin, Sara Abdelghani, Lienda Bashier Eltayeb
Abstract

Transfusion-transmitted malaria remains a critical problem for blood banks due to a symptomatic of Plasmodium infection. Malaria screening is not listed in the blood-borne pathogens screening protocol in Sudan, and the test of malaria is not routine in the blood banks for donors. We aimed to detect Plasmodium falciparum among asymptomatic blood donors in a blood bank in Sudan –Khartoum. 450 blood samples were collected and screened using microscopy, ICT, and PCR. Using the Guanidine Hydrochloride method, the genetic agent was extracted from the samples.

All examined samples were negative by microscopy and PCR for the 18SssrRNA Plasmodium falciparum gene. All donors were Sudanese males, and their ages ranged between 20-49 years old with a mean 31 ±1.45 SD. While 8 (4%) were positive by ICT, the majority of positive subjects 5 (1.1%) were in the age group between 20-29 years old, however, 192 (42.7%) of donors were in the age group 40- 49. Concerning residence, 424 (94.2%) of the participants were from Khartoum state, and 15 (3.3%) from the state of Gaziera; where one-third of them 5 (1.1%) were positive by ICT, the result was insignificant (P value=0.09). The prevalence rate of transfusion transmission malaria is 0%, although all results are negative, this does not prevent transmitted malaria through blood donors. Although the control and preventive measure for blood transfusion biosafety in Khartoum blood banks are valid and adequate, and a molecular diagnosis should run besides routine blood smear.


How to cite this article
Vancouver
Allah H M A, Abdo E, Elamin E A E, Abdelghani S, Eltayeb L B. Molecular Screening for Transfusion Transmissible P. falciparum in Asymptomatic Blood Donors in the Non-endemic Region. J Biochem Technol. 2021;12(1):6-10. https://doi.org/10.51847/D8P0P9B063
APA
Allah, H. M. A., Abdo, E., Elamin, E. A. E., Abdelghani, S., & Eltayeb, L. B. (2021). Molecular Screening for Transfusion Transmissible P. falciparum in Asymptomatic Blood Donors in the Non-endemic Region. Journal Of Biochemical Technology, 12(1),6-10. https://doi.org/10.51847/D8P0P9B063
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JOURNAL OF BIOCHEMICAL TECHNOLOGY
JOURNAL OF BIOCHEMICAL TECHNOLOGY
Journal of Biochemical Technology is a peer reviewed International Journal published by the Sevas Publishing on behalf of the Biochemical Technology Society, a Registered Charity Organization from India

AREA OF INTEREST
AREA OF INTEREST
Areas of high interest, cover new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.

FOCUS AND SCOPE
FOCUS AND SCOPE
Journal Of Biochemical Technology” Provides A Medium For The Rapid Publication Of Full-Length Articles, Mini-Reviews Of New And Emerging Products And Short Communications On All Aspects Of ...

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