Molecular docking and Evaluation of wound healing activity of stigmastatone isolated from bark of Celastrus paniculatusWilld.

Glycogen Synthase Kinase3-β (GSK3-β) in various organisms have revealed physiological roles for the enzyme in differentiation and cell fate determination. The molecular docking of GSK3-β with stigmastatone isolated from the petroleum ether extract of bark showed the inhibition constant 1.21 nM whereas, standard drug nitrofurazone showed inhibition constant of 1.35 nM. Both extract and isolated constituent were studied for their potency using excision, incision and dead space wound models in rats. In stigmastatone treated animals, epithelialization was faster with 97.01% wound contraction on 18th post wounding day. Tensile strength of incision wound was significantly increased to 592.25±1.09g after intraperitoneal administration of stigmastatone (12 mgkg-1). In dead space wound, a significant increase in weight, tensile strength and increased collagenation of granuloma tissue was observed. We reported here for the first time the wound healing property of stigmastatone that may lead to new therapeutic intervention.