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2011 Volume 3 Issue 2

Lignocellulolytic enzymes produced by tropical white rot fungi during biopulping of Acacia mangium wood chips


Abstract
Pycnoporus coccineus and Coriolus versicolor are among the tropical white rot basidiomycetes that degrade lignin selectively. In this research work, crude enzyme produced during biopulping using both fungi were extracted, filtered and assayed using specific substrates. Both the peroxidases enzyme activities and residual lignin content were measured for the incubation period of 20, 40 and 60 days of biopulping. For both fungi, manganese peroxidase is predominant and highly expressed thus shows the highest rate compared to other ligninolytic enzymes activities in all of extract preparations. After 60 days of inoculation, manganese peroxidase activities are recorded as 270.51 U/mL and 274.36 U/mL for C. versicolor and P. coccineus, respectively. On the evaluation of the lignin content after biopulping, the lignin content showed significant decreased. Wood chip biotreated with C. versicolor showed higher percentage in lignin loss (9.42%) compared to with P. coccineus (8.10%).

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JOURNAL OF BIOCHEMICAL TECHNOLOGY
JOURNAL OF BIOCHEMICAL TECHNOLOGY
Journal of Biochemical Technology is a peer reviewed International Journal published by the Sevas Publishing on behalf of the Biochemical Technology Society, a Registered Charity Organization from India

AREA OF INTEREST
AREA OF INTEREST
Areas of high interest, cover new advances in enzymatic and protein mechanims; applied molecular genetics and biotechnology; genomics and proteomics; metabolic; medical, environmental, food and agro biotechnology.

FOCUS AND SCOPE
FOCUS AND SCOPE
Journal Of Biochemical Technology” Provides A Medium For The Rapid Publication Of Full-Length Articles, Mini-Reviews Of New And Emerging Products And Short Communications On All Aspects Of ...

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This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. Keywords include, Biochemical Research: Endo/exocytosis, Trafficking, Membrane Biology, Cell Migration, Cell-Matrix Organelle Biogenesis, Cytoskeleton Proteolysis, Cell Death, Cell Cycle, Cancer, Cell Growth/Death, Differentiation, Drug Targets, Gene Therapy, Models of Disease, Proteomics, Stem Cells, Bioenergetics, Mitochondria, Free Radicals, Redox Signaling, Ion Transport/Channels, Oxidative